Viroid replication: equilibrium association constant and comparative activity measurements for the viroid-polymerase interaction
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چکیده
منابع مشابه
The viroid and viroid-like RNA database
The viroid and viroid-like RNA database is a compilation of all natural sequences published in journals or available from the GenBank and EMBL nucleotide sequence libraries. Several information regarding these RNA species such as the position of their self-catalytic domains and the open reading frame of the human hepatitits delta virus are provided. The database also includes a determination of...
متن کاملogenicity, viroid evolution, Citrus exocortis viroid, Potato spindle tuber viroid, Peach latent mosaic viroid, Chrysan- themum chlorotic mottle viroid
Viroids are autonomously replicating, small singlestranded circular RNA molecules that do not code for proteins and may cause disease in infected, susceptible plants. Viroids have the ability to induce both RNA-mediated transcriptional gene silencing (TGS) and posttranscriptional gene silencing (PTGS) in infected plants. Viroid PTGS has also been demonstrated in a wheat germ extract system. A p...
متن کاملViroid Replication: Rolling-Circles, Enzymes and Ribozymes
Viroids, due to their small size and lack of protein-coding capacity, must rely essentially on their hosts for replication. Intriguingly, viroids have evolved the ability to replicate in two cellular organella, the nucleus (family Pospiviroidae) and the chloroplast (family Avsunviroidae). Viroid replication proceeds through an RNA-based rolling-circle mechanism with three steps that, with some ...
متن کاملRna Intermediates of Viroid Replication 329
Molecular hybridization by the Northern blot technique in combina t ion with 12SI-labeled PSTV (+) RNA and S2P-labeled PSTV cDNA as probes has been applied to d e t e c t v i r o i d s p e c i f i c s e q u e n c e s in h e a l t h y and v i r o i d ( P S T V ) i n f e c t e d t o m a t o plants . Conditions are described which allow d i f f e r e n t i a t i o n of (+) and (-) viroid sequences...
متن کاملThe interaction between plant viroid-induced symptoms and RNA silencing.
A large body of evidence has lead to the suggestive proposal of a potential interplay between viroid-induced pathogenesis and RNA silencing regulatory mechanisms. A variety of techniques have been used to examine this interaction. This chapter outlines the use of a green fluorescent protein (GFP) sensor, containing viroid-specific sequence targets to study this phenomenon. In addition, a sympto...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1984
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/12.15.6231